Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 38-13] (IgM) to Human CD77 / Gb3
IHC - Frozen, Flow Cytometry
Human CD77 / Gb3
Human, Bovine (tested or 100% immunogen sequence identity)
IgM Monoclonal [38-13]
IHC - Frozen (1:1)
Flow Cytometry (1:1)
IHC - Paraffin
Specificity and Use
CD77 / Gb3 antibody was raised against daudi cell line.
Specific for the Gb3 - specific sugar sequence Gal alpha 1-4 Gal beta- 4G1c, also known as the blood group substance pk (CD77). CD77 expression amongst leucocytes is restricted to the B cell lineage, and only cells at a certain maturation stage express the antigen. In immunohistology, CD77 stains germinal centre B cells, but not those of the mantle or marginal zone. It is also expressed by follicular dendritic cells, on endothelium and on some epithelial cell types.
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing, acetone fixation is recommended for frozen sections. Flow cytometry: Use 20ul to label 5 x 105 cells in 100ul.