Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 38-13] (IgM) to Human CD77 / Gb3
Human CD77 / Gb3
Human, Bovine (tested or 100% immunogen sequence identity)
IgM Monoclonal [38-13]
Flow Cytometry (1:1)
IHC - Paraffin
Specificity and Use
CD77 / Gb3 antibody was raised against daudi cell line.
Specific for the Gb3 - specific sugar sequence Gal alpha 1-4 Gal beta- 4G1c, also known as the blood group substance pk (CD77). CD77 expression amongst leucocytes is restricted to the B cell lineage, and only cells at a certain maturation stage express the antigen. In immunohistology, CD77 stains germinal centre B cells, but not those of the mantle or marginal zone. It is also expressed by follicular dendritic cells, on endothelium and on some epithelial cell types.
Flow cytometry: Use 20ul to label 5 x 105 cells in 100ul.
Shiga Toxins Activate the NLRP3 Inflammasome Pathway To Promote Both Production of the Proinflammatory Cytokine Interleukin-1ß and Apoptotic Cell Death. Lee MS, Kwon H, Lee EY, Kim DJ, Park JH, Tesh VL, Oh TK, Kim MH. Infection and immunity. 2016 84:172-86. (Flo; Human)[Full Text Article]