Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1E6] (IgG2b,k) to Human CD71 / Transferrin Receptor
IHC, IHC - Paraffin, Western blot, ELISA
Mouse Monoclonal [clone MEM-189] (IgG1) to Human CD71 / Transferrin Receptor
Western blot, Immunoprecipitation, Flow Cytometry
Human CD71 / Transferrin Receptor
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MEM-189]
Protein A purified
Specificity and Use
KG1 human acute myelogenous leukaemia cell line.
Reacts with CD71 antigen (transferrin receptor), a 95 kD type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity.