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Anti-CD68 Antibody (clone FA-11, FITC) LS-C188201

Ordering

Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $520
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD68 Antibodies

Anti-CD68 Antibody (clone KP1) IHC-plus™ LS-B2862
Mouse Monoclonal [clone KP1] (IgG1,k) to Human CD68
Human, Monkey, Rat, Cat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-CD68 Antibody (clone ED1) IHC-plus™ LS-B4273
Mouse Monoclonal [clone ED1] (IgG1)
Rat
IHC - Paraffin, IHC - Frozen
Unconjugated
Immunohistochemistry Image
Anti-CD68 Antibody (aa312-326) LS-C312677
Rabbit Polyclonal (IgG) to Mouse CD68
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone FA-11] (IgG2a) to Mouse CD68
Mouse
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
FITC Conjugated

Details

Mouse CD68
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [FA-11]
Fluorescein. Also available Unconjugated or conjugated with FITC, Biotin, RPE.
Protein G purified
Unmodified

Applications

  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:1 - 1:10)
  • (applications tested for the base form of this product only)

Specificity and Use

CD68 antibody was raised against purified Concanavalin A acceptor glycoprotein from P815 cell line.
Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (LAMP) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeabilization. The antibody does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.09% sodium azide, 1% BSA
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. Protect from light.
For research use only.

About CD68

P34810 NM_001251 NP_001242.2

CD68 Antibody, CD68 antigen Antibody, gp110 Antibody, SCARD1 Antibody, CD68 molecule Antibody, LAMP4 Antibody, Macrophage antigen CD68 Antibody, Macrosialin Antibody

CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins.

Western blot

Western blot
Western blot of CD68 expression on J774 cells using Rat anti-Mouse CD68 with Goat anti-Rat IgG:HRP STAR72) as a detection antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Flow cytometry of mouse peritoneal macrophages cells with Rat anti-Mouse CD68:FITC . following permeablisation with Leucoperm This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Flow cytometry of mouse peritoneal macrophages with Rat anti-Mouse CD68 CD68 , following permeablisation with Leucoperm. This image was taken for the unmodified form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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