Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CD66a / CEACAM1 encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein.
CEACAM-1/CD66a in Mouse Liver. CEACAM-1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody at 5 ug/ml overnight at 4°C. Tissue was stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in bile canaliculi.
Detection of Mouse CEACAM-1/CD66a by Western Blot. Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 ug/ml of Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for CEACAM-1/CD66a between approximately 110 and 120 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of CEACAM-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody followed by Phycoerythrinconjugated Anti-Sheep IgG Secondary Antibody and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.
Requested From: United States
Date Requested: 10/21/2016