Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Fish (tested or 100% immunogen sequence identity)
IHC - Frozen
Flow Cytometry (1:1)
Specificity and Use
CD60 antibody was raised against purified CD60 antigen from PBL; 9-0-acetyl-GD3 from buttermilk.
Recognizes the human CD60 antigen, a carbohydrate structure GD3 (Neu5Aca8Neu5Aca3Galbeta4GlcCer) modified by a terminal 9-0-acetylated disialosyl group (9-O-acetyl GD3). Does not recognize 7-O-acetyl GD3 or non-acetylated GD3. CD60 has been divided into 3 sub-types depending on the acetylation present. Non acetylated GD3 is termed CD60a and 7-O-acetyl GD3 termed CD60c. 9-O-acetyl GD3 as recognized by clone M-T6004 has been designated CD60b. CD60b is present on CD8+ve helper T cells, CD4+ve memory T cells and on activated B cells. Expression has also been reported on neuroectodermal cells and in breast carcinomas. CD60b is not expressed by naive T cells. GD3 has been described as a pro-apoptotic messenger targeting mitochondria in many lymphoid and myeloid cell types. Acetylation abrogates the pro-apoptotic activity of GD3 and CD60b protects tumor cells from apoptosis.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.