Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [MEM-43]
Protein A purified
IHC - Paraffin
IHC - Frozen
Flow Cytometry (1:5 - 1:10)
Specificity and Use
CD59 antibody was raised against human CD59.
Reacts with a PI-linked glycoprotein. This antibody has been shown to stimulate NK activity. Recognizes an epitope distinct to that bound by LS-C5424.
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Flow Cytometry: 1:5-1:10. Use 10 ul to label 1E6 cells in 100ul. Immunohistochemistry (Frozen & Paraffin): Antigen retrieval using heat treatment methods prior to staining of paraffin sections is required. Sodium citrate buffer, pH 6.0, is recommended for this procedure. Positive control: Tonsil.
PBS, pH 7.2, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation.