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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2106-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human CD59 Antibody (clone MEM‑43, IHC, IF, WB) LS‑B2106
IHC‑plus™ Monoclonal Mouse anti‑Human CD59 Antibody (clone MEM‑43, IHC, IF, WB) LS‑B2106
Note: This antibody replaces LS-C11528, LS-C57173, LS-C11529
Antibody:
CD59 Mouse anti-Human Monoclonal (MEM-43) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD59 Mouse anti-Human Monoclonal (MEM-43) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD59 antibody LS-B2106 is an unconjugated mouse monoclonal antibody to human CD59. Validated for ELISA, Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD59
Synonyms
CD59 | 16.3A5 | 1F5 | 1F5 antigen | CD59 glycoprotein | EL32 | EJ16 | G344 | HRF-20 | MAC-IP | MACIF | Ly-6-like protein | MEM43 | MIRL | MEM43 antigen | MIN2 | MIN3 | MSK21 | p18-20 | Protectin | MAC-inhibitory protein | MIC11 | T cell-activating protein | CD59 antigen | EJ30 | HRF20 | Human leukocyte antigen MIC11 | Lymphocytic antigen CD59/MEM43 | MIN1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
MEM-43
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen
- Immunofluorescence
- Western blot
- Immunoprecipitation
- Flow Cytometry
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2106 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2106 was determined to be 5 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD59
Validation
Anti-CD59 antibody IHC of human skin, sweat glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CD59 antibody IHC of human skin, sweat glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD59 Antibody (clone MEM-43) for IHC, IF/Immunofluorescence, WB/Western, IP, Flow, ELISA LS-B2106 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Baboon, Monkey
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Rat
Applications:
IHC, IHC - Frozen, Western blot, Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
