Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone MEM-43] (IgG2a) to Human CD59
Immunofluorescence, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [MEM-43]
Protein A purified
Immunofluorescence (5 µg/ml)
Flow Cytometry (5 µg/ml)
Specificity and Use
CD59 antibody was raised against thymocytes and T lymphocytes.
Reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 5 microgram/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determin
PBS, 15 mM sodium azide, approx., pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation.