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Anti-CD58 Antibody (Cytoplasmic Domain) LS-C45438

Note: This antibody replaces LS-C58724

Ordering

Wt. Vol. Conc. Price
200 µg - 1 mg/ml $470
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD58 Antibodies

Anti-CD58 Antibody (clone TS2/9, Biotin) LS-C134511
Mouse Monoclonal [clone TS2/9] (IgG1) to Human CD58
Human
Flow Cytometry, Functional Assay
Biotin Conjugated
Flow Cytometry Image
Anti-CD58 Antibody (clone 2D11-B10) IHC-plus™ LS-B5386
Mouse Monoclonal [clone 2D11-B10] (IgG2a,k) to Human CD58
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a) to Human CD58
Human
Western blot, Flow Cytometry
Unconjugated

Details

Human CD58
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot
  • Flow Cytometry (1:10)

  • IHC - Paraffin
  • IHC - Frozen

Specificity and Use

CD58 antibody was raised against human erythrocytes.
Cytoplasmic Domain
CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. Reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

Packaging

0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CD58

P19256 NM_001779 NP_001770.1

CD58 Antibody, CD58 antigen Antibody, AG3 Antibody, LFA-3 Antibody, LFA3 Antibody, Surface glycoprotein LFA-3 Antibody, Surface glycoprotein lfa3 Antibody, CD58 molecule Antibody

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Requested From: United States
Date Requested: 12/11/2016

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