Human, Guinea pig, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Bovine, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
CD57 / HNK1 antibody was raised against synthetic peptide C-HVETPRNYKLRGD from an internal region of human B3GAT1 / CD57 / NK1 (NP_061114.2). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Bovine, Turkey, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish (92%); Stickleback (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186460. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human B3GAT1 / CD57 / NK1. Reported variants represent identical protein: NP_473366.1, NP_061114.2.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 38.3kD according to NP_061114.2). Recommended concentration: 0.1-0.3 ug/ml. An additional band of 17kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.