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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD57 / HNK1 Antibody (aa145‑157) LS‑C186460
HNK1 antibody LS-C186460 is an unconjugated goat polyclonal antibody to HNK1 (CD57) from human, guinea pig, hamster and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C186460-100
100 µg (0.5 mg/ml)
Unavailable

Popular CD57 / HNK1 Products

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Immunohistochemistry: CD57 Antibody - IHC of CD57 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemistry: CD57 Antibody - IHC analysis of CD57 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence

Product Description

HNK1 antibody LS-C186460 is an unconjugated goat polyclonal antibody to HNK1 (CD57) from human, guinea pig, hamster and other species. Validated for Peptide-ELISA and WB.
About CD57 / HNK1
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Q9P2W7 NM_018644 NP_061114.2

B3GAT1 Antibody, CD57 Antibody, CD57 antigen Antibody, GlcAT-P Antibody, GLCUATP Antibody, Glucuronosyltransferase P Antibody, HNK-1 Antibody, HNK1 Antibody, LEU7 Antibody, GLCATP Antibody, NK-1 Antibody, NK1 Antibody, LEU7 antigen Antibody, GlcUAT-P Antibody


Specifications

Target
Human CD57 / HNK1
Host
Goat
Reactivity
Human, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bovine, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
CD57 / HNK1 antibody was raised against synthetic peptide C-HVETPRNYKLRGD from an internal region of human B3GAT1 / CD57 / NK1 (NP_061114.2). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Bovine, Turkey, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish (92%); Stickleback (85%).
Blocking Peptide
LS-E28037 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186460. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa145-157
Specificity
Human B3GAT1 / CD57 / NK1. Reported variants represent identical protein: NP_473366.1, NP_061114.2.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 38.3kD according to NP_061114.2). Recommended concentration: 0.1-0.3 ug/ml. An additional band of 17kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/25/2018

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