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Anti-CD57 / HNK1 Antibody (aa145-157) LS-C186460

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD57 / HNK1 Antibodies

Anti-CD57 / HNK1 Antibody (PE, Cy5) LS-C13837
Mouse Monoclonal (IgM) to Human CD57 / HNK1
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
PE, Cy5 Conjugated
Anti-CD57 / HNK1 Antibody (clone NK-1) IHC-plus™ LS-B7765
Mouse Monoclonal [clone NK-1] (IgM,k) to Human CD57 / HNK1
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD57 / HNK1 Antibody LS-C180182
Rabbit Polyclonal to Human CD57 / HNK1
Human, Mouse
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human CD57 / HNK1
Human, Guinea pig, Hamster, Horse, Pig, Rabbit
Western blot, ELISA
Unconjugated

Details

Human CD57 / HNK1
Goat
Human, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bovine, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:64000)

Specificity and Use

CD57 / HNK1 antibody was raised against synthetic peptide C-HVETPRNYKLRGD from an internal region of human B3GAT1 / CD57 / NK1 (NP_061114.2). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Bovine, Turkey, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish (92%); Stickleback (85%).
LS-E28037 - Lyophilized - 100 µg - $145.00
aa145-157
Human B3GAT1 / CD57 / NK1. Reported variants represent identical protein: NP_473366.1, NP_061114.2.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 38.3kD according to NP_061114.2). Recommended concentration: 0.1-0.3 ug/ml. An additional band of 17kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CD57 / HNK1

Q9P2W7 NM_018644 NP_061114.2

B3GAT1 Antibody, CD57 Antibody, CD57 antigen Antibody, GlcAT-P Antibody, GLCUATP Antibody, Glucuronosyltransferase P Antibody, HNK-1 Antibody, HNK1 Antibody, LEU7 Antibody, GLCATP Antibody, NK-1 Antibody, NK1 Antibody, LEU7 antigen Antibody, GlcUAT-P Antibody

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin.

Western blot

Western blot
B3 ugAT1 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/4/2016

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