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Anti-CD55 Antibody (clone 67) LS-C11548

Note: This antibody replaces LS-C57446, LS-C11547


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $250
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Mouse Monoclonal [clone 67] (IgG1) to Human CD55
IHC - Frozen, Flow Cytometry


Human CD55
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [67]
Tissue culture supernatant


  • IHC - Frozen (1:100 - 1:1000)
  • Flow Cytometry (1:10 - 1:25)

Specificity and Use

CD55 antibody was raised against k562 cells.
Recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak. CD55 is the complement regulatory protein, decay accelerating factor (DAF). Human CD55 is a ~70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-hemopoietic tissues, particularly epithelium and is specifically found at the fetal-maternal interface in placenta and. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. We do not recommend the use of this reagent to stain erythrocytes.


PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CD55

P08174 NM_000574 NP_000565.1

CD55 Antibody, CD55 antigen Antibody, Decay-accelerating factor Antibody, TC Antibody, CR Antibody, CROM Antibody, DAF Antibody

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.

Requested From: United States
Date Requested: 10/25/2016

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