Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone BRIC126] (IgG2b) to Human CD47
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [BRIC126]
Protein G purified from tissue culture supernatant
IHC - Frozen
Specificity and Use
CD47 antibody was raised against human erythrocytes.
Recognizes the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Tissue culture supernatant
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.