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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD45RO Antibody (clone UCHL1, FITC) LS‑C45114
Note: This antibody replaces LS-C58472, LS-C21906
CD45RO antibody LS-C45114 is an FITC-conjugated mouse monoclonal antibody to human CD45RO. Validated for Flow, IHC and IP.
Catalog
Size
Price
LS-C45114-100
100 µg (0.1 mg/ml)
Unavailable

Popular CD45RO Products

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO, T cell antibody LS-C95314.
Species: Human
Applications: IHC, IHC - Paraffin
Staining of normal human lysed whole blood with PE anti-human CD45RA (HI100) (LS-C106068) and APC Mouse IgG2a, kappa Isotype Control (left) or APC anti-human CD45RO (UCHL1) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
IHC testing of CD45RO antibody and FFPE human tonsil.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

Product Description

CD45RO antibody LS-C45114 is an FITC-conjugated mouse monoclonal antibody to human CD45RO. Validated for Flow, IHC and IP.
About CD45RO
Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). The CD45 family consists of multiple members that are all products of a single complex gene.

Specifications

Target
Human CD45RO
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [UCHL1]
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Cy5, RPE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD45RO antibody was raised against human IL-2 dependent T cells.
Specificity
Recognizes the low molecular weight isoform (180kD) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood, UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.
Presentation
PBS, pH 7.2, 0.09% sodium azide, 1% BSA.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45RO antibody IHC of human spleen, t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7300 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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