Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone RA3-6B2] (IgG2a,k) to Mouse CD45R
IHC - Paraffin, IHC - Frozen, Flow Cytometry
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [RA3-6B2]
IHC - Paraffin
IHC - Frozen
Specificity and Use
CD45R antibody was raised against mouse CD45R
The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.
Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). The CD45 family consists of multiple members that are all products of a single complex gene.
Staining of C57Bl/6 splenocytes with 0.5 ug of Purified Rat IgG2a isotype control (open histogram) or 0.5 ug of Purified anti-mo/hu B220 (RA3-6B2) (colored histogram) followed by FITC Anti-Rat IgG. Cells in the lymphocyte gate were used for analysis.