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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD45 / LCA Antibody (Internal) IHC‑plus™ LS‑A9435
LCA antibody LS-A9435 is an unconjugated rabbit polyclonal antibody to human LCA (CD45). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A9435-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

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Product Description

LCA antibody LS-A9435 is an unconjugated rabbit polyclonal antibody to human LCA (CD45). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About CD45 / LCA
CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. P08575 NM_002838 NP_002829.3

PTPRC Antibody, B220 Antibody, CD45R Antibody, CD45 Antibody, gp180 Antibody, Leukocyte common antigen Antibody, L-CA Antibody, Leucocyte common antigen Antibody, LY5 Antibody, T200 Antibody, T200 glycoprotein Antibody, T200 leukocyte common antigen Antibody, CD45 antigen Antibody, LCA Antibody


Specifications

Target
Human CD45 / LCA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD45 / LCA antibody was raised against synthetic 16 amino acid peptide from internal region of human CD45. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (88%).
Blocking Peptide
LS-E29647 - Liquid - 50 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-A9435. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human CD45. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A9435 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9435 was determined to be 2.5-5 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.

Immunohistochemistry

Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.


Requested From: 
Date Requested: 6/21/2018

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