Mouse CD45 / LCA

Rat

Mouse (tested or 100% immunogen sequence identity)

Human, Rat

IgG1 Monoclonal

IBL-3/16

Unconjugated

Protein G purified

Unmodified

Purified B cells from mouse lymph nodes.

Recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kD single pass type I transmembrane glycoprotein belonging to the protein -tyrosine phosphatase family. CD45 has two domains and two domains. Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Is expected to recognize all isoforms of murine CD45. Has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques.

• IHC
• IHC - Frozen (1:50 - 1:100)
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry (1:50 - 1:100)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

PBS, 0.09% Sodium Azide, 0.1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08575 NCBI: NM_002838 NP_002829.3