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Anti-CD45 / LCA Antibody (clone BHPT-1, Biotin) LS-C140358

Ordering

Wt. Vol. Conc. Price
- 100 tst - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD45 / LCA Antibodies

Anti-CD45 / LCA Antibody (PE) LS-C127700
Mouse Monoclonal (IgG1) to Sheep CD45 / LCA
Sheep, Bovine, Goat
Flow Cytometry
PE Conjugated
Anti-CD45 / LCA Antibody (Internal) IHC-plus™ LS-A9435
Rabbit Polyclonal to Human CD45 / LCA
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone BHPT-1] (IgG1) to Human CD45 / LCA
Human
Flow Cytometry
Biotin Conjugated

Details

Human CD45 / LCA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [BHPT-1]
Biotin
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD45 / LCA antibody was raised against peripheral blood monocytes from rheumatoid arthritis patient.
Human PTPRC / CD45
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.

About CD45 / LCA

P08575 NM_002838 NP_002829.3

PTPRC Antibody, B220 Antibody, CD45R Antibody, CD45 Antibody, gp180 Antibody, Leukocyte common antigen Antibody, L-CA Antibody, Leucocyte common antigen Antibody, LY5 Antibody, T200 Antibody, T200 glycoprotein Antibody, T200 leukocyte common antigen Antibody, CD45 antigen Antibody, LCA Antibody

CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.

Requested From: United States
Date Requested: 12/8/2016

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