Human CD45 / LCA

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

2B11 + PD7/26

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.

Isolated neoplastic cells from T cell lymphoma were used as the immunogen for clone 2B11 and human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for clone PD7/26.

Human CD45

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This antibody cocktail contains an antibody which binds to all CD45 leukocyte common antigen (LCA) family isoforms (clone 2B11), and an antibody specific to the CD45RB isoform (clone PD7/26). CD45 is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of the CD45 isoforms have protein phosphatase activity and are involved in regulation of transmembrane signals. antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this antibody cocktail is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody cocktail detects CD45 expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

1X PBS

Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08575 NCBI: NM_002838 NP_002829.3