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Anti-CD45 / LCA Antibody (aa1029-1249, clone 3G4) IHC-plus™ LS-B2941

Note: This antibody replaces LS-C93717

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD45 / LCA Antibodies

Anti-CD45 / LCA Antibody (PE) LS-C127700
Mouse Monoclonal (IgG1) to Sheep CD45 / LCA
Sheep, Bovine, Goat
Flow Cytometry
PE Conjugated
Anti-CD45 / LCA Antibody (Internal) IHC-plus™ LS-A9435
Rabbit Polyclonal to Human CD45 / LCA
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3G4] (IgG2a) to Human CD45 / LCA
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CD45 / LCA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3G4]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:3000)
  • ELISA

Specificity and Use

CD45 / LCA antibody was raised against recombinant human CD45 (1029-1249aa) purified from E. coli.
aa1029-1249
Immunohistochemistry: LS-B2941 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2941 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CD45 / LCA

P08575 NM_002838 NP_002829.3

PTPRC Antibody, B220 Antibody, CD45R Antibody, CD45 Antibody, gp180 Antibody, Leukocyte common antigen Antibody, L-CA Antibody, Leucocyte common antigen Antibody, LY5 Antibody, T200 Antibody, T200 glycoprotein Antibody, T200 leukocyte common antigen Antibody, CD45 antigen Antibody, LCA Antibody

CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.

Immunohistochemistry

Immunohistochemistry
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2941 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2941 concentration 10 ug/ml.

Western blot

Western blot
Cell lysates of Jurkat(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/3/2016

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