Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Mouse Monoclonal (IgG1) to Human CD44
Human, Mouse, Rat
Western blot, Immunoprecipitation
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Tissue culture supernatant
Western blot (1:1000)
Specificity and Use
CD44 antibody was raised against immunizing BALB/c mice with purified recombinant CD44 protein.
Recognizes human CD44. Species cross-Reactivity: mouse, rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X. TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol, 0.02% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.