Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin
IHC - Frozen
Western blot (1:1000)
Specificity and Use
CD44 antibody was raised against recombinant human CD44var(v3-v10).
Recognizes epitopes encoded by exons v3-v10 of human variant CD44.
Suitable for use in Immunohistochemistry and Western Blot. Western Blot: 1:1000. Immunohistochemistry (Frozen): 50 ug/ml. Use alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedure or the three stage immunoperoxidase technique on acetone fixed frozen sections. Immunohistochemistry (Paraffin): 20 ug/ml after 1x5 min microwave treatment. Use of biotinylated swine anti-goat, mouse, rabbit immunoglobulins is recommended. Blocking should be done with swine serum.
PBS, pH 7.3.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.