Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Goat Polyclonal to Human CD44
IHC, IHC - Paraffin, IHC - Frozen
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (20 µg/ml)
IHC - Frozen (50 µg/ml)
Specificity and Use
CD44 antibody was raised against recombinant human CD44v3-v10.
Recognizes epitopes encoded by exons v3-v10 on the variant portion of human CD44. CD44 is a ubiquitously expressed type 1 transmembrane glycoprotein and proteoglycan, otherwise known as phagocytic glycoprotein 1 (pgp 1), acting as a receptor for the major extracellular matrix component hyaluronate, and also binds other ligands including collagen and matrix metalloproteinases. CD44 plays a role in cell-cell interactions, cell adhesion, the activation and homing of lymphocytes, cell migration, haematopoiesis and metastasis, and exists as a number of isoforms generated by the alternative RNA splicing of at least 10 variant exons.
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.