Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
CD44 in Mouse Splenocytes. CD44 was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse/ Rat CD44 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces.
Detection of Mouse and Rat CD44 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse spleen tissue, mouse lymph node tissue, and rat brain tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse/Rat CD44 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for CD44 at approximately 80 to 100 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of CD44 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Sheep Anti-Mouse/Rat CD44 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.
Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse/Rat CD44 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.