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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD40 Antibody IHC‑plus™ LS‑B9072
Note: This antibody replaces LS-C5089
CD40 antibody LS-B9072 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, ICC, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9072-100
100 µg (1 mg/ml)
Unavailable

Popular CD40 Products

Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1956 concentration 10 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Flow Cytometry
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Horse
Applications: IHC, IHC - Paraffin, Western blot
Anti-CD40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2596 concentration 10 ug/ml.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Anti-CD40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5282 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
IHC of paraffin-embedded Human liver tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CD40 antibody LS-B9072 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, ICC, IHC and IP. Tested on 20 paraffin-embedded human tissues.
About CD40
CD40 is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. P25942 NM_001250 NP_001241.1

CD40 Antibody, B-cell surface antigen CD40 Antibody, B cell-associated molecule Antibody, B cell surface antigen CD40 Antibody, CD40 antigen Antibody, CD40 type II isoform Antibody, p50 Antibody, Bp50 Antibody, CD40L receptor Antibody, CDW40 Antibody, TNFRSF5 Antibody


Specifications

Target
Human CD40
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • ICC (1:10)
  • Immunoprecipitation
  • Flow Cytometry (20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Recognizes human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
Usage
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Flow Cytometry: 20 ug/ml. Use 10 ul to lane 1E6 cells in 100ul. Immunohistochemistry (Frozen and paraffin): 1-10 ug/ml. Requires protein digestion pre-treatment of paraffin embedded sections prior to staining. Immunocytochemistry: 1:10 ug/ml. Positive control: Human tonsil.
Presentation
TBS, pH 7.4, 0.09% sodium azide
Storage
Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Requested From: 
Date Requested: 6/22/2018

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