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Anti-CD40 Antibody (clone LOB7/6) IHC-plus™ LS-B2596

Note: This antibody replaces LS-C11546, LS-C57442

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD40 Antibodies

Anti-CD40 Antibody (clone HB14) IHC-plus™ LS-B1956
Mouse Monoclonal [clone HB14] (IgG1,k) to Human CD40
Human, Monkey
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD40 Antibody (aa258-277) LS-C63147
Rabbit Polyclonal to Human CD40
Human, Mouse, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone LOB7/6] (IgG2a) to Human CD40
Human, Dog
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CD40
Mouse
Human, Dog (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [LOB7/6]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1 - 10 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (20 µg/ml)

Specificity and Use

Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
Immunohistochemistry: LS-B2596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2596 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.

Packaging

Tris buffered saline, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.
For research use only.

Publications (1)

1
The candidate immunotherapeutical target, the receptor for hyaluronic acid-mediated motility, is associated with proliferation and shows prognostic value in B-cell chronic lymphocytic leukemia. Giannopoulos K, Mertens D, Bhler A, Barth TF, Idler I, Mller P, Krber A, Greiner J, Chocholska S, Dmoszyska A, Roliski J, Dhner H, Stilgenbauer S, Schmitt M. Leukemia. 2009 23:519-27. (IHC-P; Human) [PubMed:19092852]

About CD40

P25942 NM_001250 NP_001241.1

CD40 Antibody, B-cell surface antigen CD40 Antibody, B cell-associated molecule Antibody, B cell surface antigen CD40 Antibody, CD40 antigen Antibody, CD40 type II isoform Antibody, p50 Antibody, Bp50 Antibody, CD40L receptor Antibody, CDW40 Antibody, TNFRSF5 Antibody

CD40 is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Immunohistochemistry

Immunohistochemistry
Anti-CD40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2596 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/2/2016

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