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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD40 Antibody (clone HM40‑3) LS‑C106071
CD40 antibody LS-C106071 is an unconjugated hamster monoclonal antibody to mouse CD40. Validated for Flow.
Catalog
Size
Price
LS-C106071-50
50 µg
Unavailable
LS-C106071-100
100 µg
Unavailable
LS-C106071-500
500 µg (0.5 mg/ml)
Unavailable

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Product Description

CD40 antibody LS-C106071 is an unconjugated hamster monoclonal antibody to mouse CD40. Validated for Flow.
About CD40
CD40 is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. P25942 NM_001250 NP_001241.1

CD40 Antibody, B-cell surface antigen CD40 Antibody, B cell-associated molecule Antibody, B cell surface antigen CD40 Antibody, CD40 antigen Antibody, CD40 type II isoform Antibody, p50 Antibody, Bp50 Antibody, CD40L receptor Antibody, CDW40 Antibody, TNFRSF5 Antibody


Specifications

Target
Mouse CD40
Host
Hamster
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal [HM40-3]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
CD40 antibody was raised against mouse CD40
Usage
The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 500 mM sodium chloride, 0.09% sodium azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.
Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.
Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.
Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.
Staining of BALB/c splenocytes with 0.5 ug Purified Armenian Hamster IgG isotype control (open histogram) or 0.5 ug Purified anti-mouse/rat CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG. Total viable cells were used for analysis.


Requested From: 
Date Requested: 6/18/2018

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