Target
Rat CD4
Synonyms
CD4 | CD4 antigen | CD4 receptor | CD4mut | CD4 antigen (p55) | CD4 molecule
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
R. Phycoerythrin
Immunogen
Rat thymocyte membrane glycoproteins (DOMAIN 1)
Specificity
Recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. Inhibits proliferation and IL-2 production in the MLR reaction. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (3) This product is routinely tested in flow cytometry on rat splenocytes.
Applications
- Flow Cytometry (1:1 - 1:10)
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee