Mouse CD4

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

RM4-5

R. Phycoerythrin

Protein G purified

Unmodified

BALB/c mouse thymocytes

Detects mouse CD4, a 55 kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. The RM4-5 antibody is useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

• Flow Cytometry

Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA

Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01730 NCBI: NM_000616 NP_000607.1