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Catalog Number Size Price
LS-C21539-100 100 µg $429 
LS-C21539-500 500 µg $666 
Antibody

Monoclonal Rat anti‑Mouse CD3E Antibody (clone YCD3‑1, FITC, IHC) LS‑C21539

Monoclonal Rat anti‑Mouse CD3E Antibody (clone YCD3‑1, FITC, IHC) LS‑C21539

Antibody:
CD3E Rat anti-Mouse Monoclonal (FITC) (YCD3-1) Antibody
Application:
IHC, IP, Flo
Reactivity:
Mouse
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$429
LS-C21539-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD3E Rat anti-Mouse Monoclonal (FITC) (YCD3-1) Antibody
Application:
IHC, IP, Flo
Reactivity:
Mouse
Format:
FITC, Unmodified
Other formats:

Specifications

Description
CD3E antibody LS-C21539 is an FITC-conjugated rat monoclonal antibody to mouse CD3E. Validated for Flow, IHC and IP.
Target
Mouse CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
YCD3-1
Conjugations
FITC. Also available conjugated with FITC, APC, PE, Cy5, FITC, Biotin, PE, APC, PE, Cy5, Cy5, Biotin, PE, APC, PE, Cy5, FITC, Biotin, PE, PE, Cy5, FITC, Biotin, PE, FITC, Biotin, PE, APC, PE, Cy5, Cy5, PE, Cy5.5, PE, Cy7, Biotin.
Purification
Purified
Modifications
Unmodified
Specificity
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
Applications
  • IHC
  • Immunoprecipitation
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Identification and enumeration of CD3+ cells by flow cytometry. Immunoprecipitation. In vitro depletion of CD3+ cells. In vitro activation of T cells (accessory cells must be provided). Immunohistochemistry tested with mouse samples fixed in PLP and then paraffin-embedded (see Whiteland etal (1995) J Histochem Cytochem 43:313). Flow Cytometry: 3 ug/1E6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024