Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Human thymocytes/Sezary T cells
Specificity
Antibody UCHT1 recognizes the 20 kd epsilon chain (3) of the CD3 molecule complex (1, 2).
Applications
- Western blot
- Flow Cytometry
Usage
Five x 105 human HPB-MLT cultured tumor cells were washed and and incubated 45 minutes on ice with 80 ul of anti-CD3 Fab at 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
Sterile Solution 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, No preservatives added
Storage
Store at 4°C. Do not freeze. Open under aseptic conditions.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee