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Anti-CD3E Antibody (clone M2AB, PE, Cy5) LS-C140477

Ordering

Wt. Vol. Conc. Price
- 100 tst - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD3E Antibodies

Anti-CD3E Antibody (Azide-free) LS-C78117
Mouse Monoclonal (IgG1) to Human CD3E
Human
IHC, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Anti-CD3E Antibody (Biotin) LS-C78118
Mouse Monoclonal (IgG1) to Human CD3E
Human
IHC, Flow Cytometry
Biotin Conjugated
Anti-CD3E Antibody (clone 3E10) LS-C174661
Mouse Monoclonal [clone 3E10] (IgG2b) to Human CD3E
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone M2AB] (IgG1) to Human CD3E
Human
Flow Cytometry
PE, Cy5 Conjugated

Details

Human CD3E
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [M2AB]
Phycoerythrin, Cy5
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

Human CD3G
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: Use as above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.

About CD3E

NM_000733 NP_000724.1

CD3E Antibody, CD3e antigen Antibody, CD3-epsilon Antibody, T-cell receptor epsilon chain Antibody, TCRE Antibody, T-cell receptors epsilon chain Antibody, T3E Antibody, CD3 Epsilon Antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Requested From: United States
Date Requested: 12/9/2016

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