Human CD3E

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

M2AB

Biotin. Also available Unconjugated.

Protein A/G purified

Unmodified

Human thymocytes

Human PGR / Progesterone Receptor

• Flow Cytometry
• Applications tested for the base form of this product only

PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2° to 8o C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use as above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1