LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-CD3E Antibody (Biotin) LS-C45013

Note: This antibody replaces LS-C58257


Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $225
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Alternate Forms of this Antibody

LS-C45012 Unconjugated
LS-C188377 Cy5, RPE Conjugated

Most Popular CD3E Antibodies

Anti-CD3E Antibody (Azide-free) LS-C78117
Mouse Monoclonal (IgG1) to Human CD3E
IHC, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Anti-CD3E Antibody (Biotin) LS-C78118
Mouse Monoclonal (IgG1) to Human CD3E
IHC, Flow Cytometry
Biotin Conjugated
Anti-CD3E Antibody (clone 3E10) LS-C174661
Mouse Monoclonal [clone 3E10] (IgG2b) to Human CD3E
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Hamster Monoclonal (IgG) to Mouse CD3E
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Biotin Conjugated


Mouse CD3E
Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal
Protein G purified


  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • (applications tested for the base form of this product only)

Specificity and Use

CD3E antibody was raised against h-2K b - specific mouse cytotoxic T lymphocyte clone BM10-37.
Detects CD3 epsilon (CD3e), a 20kD transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3e is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.


PBS, pH 7.2, 0.09% sodium azide, 1% BSA.
For research use only.

About CD3E

NM_000733 NP_000724.1

CD3E Antibody, CD3e antigen Antibody, CD3-epsilon Antibody, T-cell receptor epsilon chain Antibody, TCRE Antibody, T-cell receptors epsilon chain Antibody, T3E Antibody, CD3 Epsilon Antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Requested From: United States
Date Requested: 10/25/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number