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Anti-CD34 Antibody (clone QBEnd/10) IHC-plus™ LS-B5552

Note: This antibody replaces LS-C134827

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD34 Antibodies

Anti-CD34 Antibody (Extracellular Domain) LS-C35556
Goat Polyclonal (IgG) to Pig CD34
Pig
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone QBEnd/10] (IgG1) to Human CD34
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CD34
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [QBEnd/10]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:800)
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

CD34 antibody was raised against human endothelial vesicles.
The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on hematopoietic cells and on the established myeloid leukemic cell line KG1A. QBEND-10 recognizes the Class II epitope on CD34. This antibody does not cross-react with tested species such as sheep, cow, dog and rat. Clone is reported to cross react with Rhesus Monkey (2). Immunogen: human endothelial vesicles.
The antibody can be used for flow cytometry, immunohistochemistry on frozen and paraffin embedded section, immunoprecipitation and Western blotting. Instructions for use Flow cytometry: Use 10 ul antibody (1:100-1:200 dilution) to label 106 cells in 100 ul Immunohistochemistry: This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended dilution: 1:500 - 1:1000 for immunohistochemistry on paraffin sections. Recommended positive control tissue: Tonsil, bone marrow.

Packaging

PBS, 0.09% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CD34

P28906 NM_001773 NP_001764.1

CD34 Antibody, CD34 molecule Antibody, CD34 antigen Antibody

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells. CD34 is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes.

Immunohistochemistry

Immunohistochemistry
Anti-CD34 antibody IHC of human kidney, glomeruli. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5552 dilution 1:800.
 

Requested From: United States
Date Requested: 12/5/2016

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