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Anti-CD34 Antibody (clone MEC14.7) IHC-plus™ LS-B2425

Note: This antibody replaces LS-C4946

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

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Rat Monoclonal [clone MEC14.7] (IgG2a) to Mouse CD34
Mouse, Human
IHC - Paraffin, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated

Details

Mouse CD34
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [MEC14.7]
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunoprecipitation (2 - 10 µg/ml)
  • Flow Cytometry (1:25 - 1:50)
  • (applications tested for the base form of this product only)

Specificity and Use

CD34 antibody was raised against mouse CD34
Recognizes the mouse CD34 cell surface glycoprotein. mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.
Immunohistochemistry: LS-B2425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2425 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.09% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CD34

P28906 NM_001773 NP_001764.1

CD34 Antibody, CD34 molecule Antibody, CD34 antigen Antibody

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells. CD34 is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes.

Immunohistochemistry

Immunohistochemistry
Anti-CD34 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2425 concentration 10 ug/ml.

Requested From: 
Date Requested: 4/27/2017

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