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Anti-CD34 Antibody (clone CS37, PE) LS-C140340

Ordering

Wt. Vol. Conc. Price
- 100 tst - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD34 Antibodies

Anti-CD34 Antibody (Extracellular Domain) LS-C35556
Goat Polyclonal (IgG) to Pig CD34
Pig
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone CS37] (IgG1) to Human CD34
Human
Flow Cytometry
PE Conjugated

Details

Human CD34
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [CS37]
Phycoerythrin
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD34 antibody was raised against human cell line KG-1a.
Human CD34
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.

About CD34

P28906 NM_001773 NP_001764.1

CD34 Antibody, CD34 molecule Antibody, CD34 antigen Antibody

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells. CD34 is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes.

Requested From: United States
Date Requested: 12/3/2016

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