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Anti-CD33 Antibody (clone 2D12-G4) LS-C196854

Catalog Size Price
LS-C196854-100 100 µg Unavailable

Related Products

159 CD33 Antibodies

Most Popular CD33 Antibodies

Anti-CD33 Antibody (aa12-39) LS-C97823
Rabbit Polyclonal to Human CD33
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Formalin-fixed and paraffin-embedded human lymph with CD33 (SIGLEC3) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-CD33 Antibody (clone WM-53 (WM53), FITC) LS-C106091
Mouse Monoclonal [clone WM-53 (WM53)] (IgG1,k) to Human CD33
Human
Flow Cytometry
FITC Conjugated
Staining of granulocyte gates were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD33 Antibody (clone HIM3-4, PE) LS-C106519
Mouse Monoclonal [clone HIM3-4] (IgG1,k) to Human CD33
Human
Flow Cytometry
PE Conjugated
Surface staining of normal human peripheral blood cells with anti-human CD33 (HIM3-4) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD33 Antibody IHC-plus™ LS-B11919
Rabbit Polyclonal (IgG) to Human CD33
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot
Unconjugated
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-CD33 Antibody (clone CD33 1C7/1) LS-C348227
Mouse Monoclonal [clone CD33 1C7/1] (IgG1) to Human CD33
Human
Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 2D12-G4] (IgG2a,k) to Human CD33
Human
Western blot, ELISA, RNA interference
Unconjugated

Details

Human CD33
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2D12-G4]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

CD33 antibody was raised against cD33 (AAH28152.1, aa1-364 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CD33
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About CD33

P20138 NM_001772 NP_001763.3

CD33 Antibody, CD33 antigen Antibody, CD33 molecule Antibody, gp67 Antibody, SIGLEC-3 Antibody, p67 Antibody, CD33 antigen (gp67) Antibody, SIGLEC3 Antibody

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Western blot

Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4.Lane 1: CD33 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4.Lane 1: CD33 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.

Western blot

CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60.
CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60.

RNA interference

Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: 
Date Requested: 5/26/2018

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