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Catalog Number Size Price
LS-B11077-50 50 µl (1.09 mg/ml) $460 
CD32A Antibody - Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
CD32A Antibody - Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD32A Antibody - Anti-FCGR2A mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
CD32A Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
CD32A Antibody - Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CD32A Antibody - Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
CD32A Antibody - Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD32A Antibody - Anti-FCGR2A mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
CD32A Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
CD32A Antibody - Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human CD32A Antibody (clone 13D7, IHC, IF, WB) LS‑B11077

IHC‑plus™ Monoclonal Mouse anti‑Human CD32A Antibody (clone 13D7, IHC, IF, WB) LS‑B11077

Note: This antibody replaces LS-C114743
Antibody:
CD32A Mouse anti-Human Monoclonal (13D7) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11077-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD32A Mouse anti-Human Monoclonal (13D7) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD32A antibody LS-B11077 is an unconjugated mouse monoclonal antibody to human CD32A. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD32A
Synonyms
FCGR2A | CD32 | CD32 antigen | CDw32 | FCG2 | FcGR | FCGR2A1 | FcRII-a | Fc-gamma RII-a | Fc-gamma-RIIa | FcRII-c | IgG Fc receptor II-a | Igg fc receptor ii-c | Fc-gamma rii-c | FCGR2 | Igg fc receptors ii-c | CD32A | IGFR2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
13D7
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Specificity
Human CD32A / CD32
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD32A
P12318 NM_021642 NP_067674.2

Validation

CD32A Antibody - Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

CD32A Antibody - Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CD32A Antibody (clone 13D7) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B11077 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024