Human CD300A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

MEM-260

Unconjugated

Protein A purified

Unmodified

Human HPB acute lymphoid leukaemia cell line.

Recognizes human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), a 60kD transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells. Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

• Western blot
• Flow Cytometry

Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. Western Blot: LS-C45034 detects a band of approximately 60kD in HPB cell lysates under non-reducing conditions.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UGN4 NCBI: NM_007261 CAB52291.1