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Anti-CD3E Antibody (aa156-168) IHC-plus™ LS-B3320

Note: This antibody replaces LS-C50360

Ordering

Wt. Vol. Conc. Price
- 250 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CD3E Antibody LS-B3320 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CD3E antibody IHC of human thymus.
Immunohistochemistry
Anti-CD3E antibody IHC of human spleen.
Western blot
Western blot of CD3E antibody

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Rabbit Polyclonal to Human CD3E
Human, Baboon, Monkey, Cat, Dog, Horse
IHC - Paraffin
Unconjugated

Details

Human CD3E
Rabbit
Human, Baboon, Monkey, Cat, Dog, Horse (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:50)

Specificity and Use

CD3E antibody was raised against a synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein.
aa156-168
Immunohistochemistry: LS-B3320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3320 was determined to be 1:50.

Packaging

10 mM PBS, 1% BSA buffer pH 7.4 with 0.1% sodium azide.
Store at 2°C to 8°C degrees. Do not freeze.
For research use only.

About CD3E

NM_000733 NP_000724.1

CD3E Antibody, CD3e antigen Antibody, CD3-epsilon Antibody, T-cell receptor epsilon chain Antibody, TCRE Antibody, T-cell receptors epsilon chain Antibody, T3E Antibody, CD3 Epsilon Antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Immunohistochemistry

Immunohistochemistry
Anti-CD3E antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3320 dilution 1:50.

Immunohistochemistry

Immunohistochemistry
Anti-CD3E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3320 dilution 1:50.

Western blot

Western blot
Western blot of CD3E antibody
 

Requested From: United States
Date Requested: 8/1/2015

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