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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD3 Antibody LS‑C78143
Antibody
CD3 antibody LS-C78143 is an unconjugated rat monoclonal antibody to mouse CD3. Validated for Flow and IHC.
Catalog
Size
Price
LS-C78143-100
100 µg
Unavailable

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Product Description

CD3 antibody LS-C78143 is an unconjugated rat monoclonal antibody to mouse CD3. Validated for Flow and IHC.
About CD3
The CD3 (cluster of differentiation 3) T-cell co-receptor is a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3gamma chain, a CD3delta chain, and two CD3epsilon chains. These chains associate with a molecule known as the T-cell receptor (TCR) and the zeta-chain to generate an activation signal in T lymphocytes. The TCR, zeta-chain, and CD3 molecules together comprise the TCR complex.

Specifications

Target
Mouse CD3
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Recognizes the mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells. NB. For optimal staining incubations should be performed at room temperature.
Usage
Immunohistochemistry (frozen). Flow Cytometry. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1E6 cells in 100ul. Applications: Immunohistochemistry (frozen). Flow Cytometry.
Presentation
PBS, pH 7.2, 0.1% sodium azide.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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