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| Catalog Number | Size | Price |
|---|---|---|
| LS-C58677-0.1 | 0.1 mg (1 mg/ml) | $395 |
Monoclonal Mouse anti‑Rat CD3 Antibody (clone 1F4, IHC) LS‑C58677
Monoclonal Mouse anti‑Rat CD3 Antibody (clone 1F4, IHC) LS‑C58677
Note: This antibody replaces LS-C58672
Antibody:
CD3 Mouse anti-Rat Monoclonal (1F4) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD3 Mouse anti-Rat Monoclonal (1F4) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
CD3 antibody LS-C58677 is an unconjugated mouse monoclonal antibody to rat CD3. Validated for Flow, IHC and IP.
Host
Mouse
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgM
Monoclonal
Clone
1F4
Conjugations
Unconjugated.
Also available conjugated with FITC.
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
Specificity
Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
Applications
- IHC
- IHC - Paraffin (1:10)
- IHC - Frozen (1:10 - 1:25)
- Immunoprecipitation
- Flow Cytometry (1:10 - 1:25)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Frozen, Flow Cytometry, Functional Assay
Species:
Human
Applications:
Western blot, Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
