(tested or 100% immunogen sequence identity)
Protein G purified
- Flow Cytometry (1:50 - 1:100)
- Functional Assay
CD28 antibody was raised against alternate immunization with A20 cells expressing mCD28 and recombinant mCD28Ig.
Recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Flow Cytometry: Use10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.