Target
Mouse CD28
Synonyms
CD28 | CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 molecule
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
R. Phycoerythrin
Purification
Affinity purified
Immunogen
Alternate immunization with A20 cells expressing mCD28 and recombinant mCD28Ig
Specificity
Recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Applications
- Flow Cytometry (1:1 - 1:10)
Usage
Flow Cytometry: Use10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 5% Sucrose
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee