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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD28 Antibody (clone ANC28.1/5D10, Fab'2) LS‑C134366
Antibody
CD28 antibody LS-C134366 is an unconjugated mouse monoclonal antibody to human CD28. Validated for ELISA, Flow and Func.
Catalog
Size
Price
LS-C134366-100
100 µg (1 mg/ml)
Unavailable

Popular CD28 Products

Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry, ELISA
Antibody
Species: Human
Applications:
Anti-CD28 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunoprecipitation, Flow Cytometry, Functional Assay

Product Description

CD28 antibody LS-C134366 is an unconjugated mouse monoclonal antibody to human CD28. Validated for ELISA, Flow and Func.
About CD28
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. P10747 NM_006139 NP_006130.1

CD28 Antibody, CD28 antigen Antibody, CD28 antigen (Tp44) Antibody, Tp44 Antibody, CD28 molecule Antibody


Specifications

Target
Human CD28
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [ANC28.1/5D10]
Conjugations
Unconjugated
Purification
Gel filtration
Modifications
Fab'2
Applications
  • Flow Cytometry
  • ELISA
  • Functional Assay
Failed Applications
  • Western blot
Immunogen
CD28 antibody was raised against recombinant Human CD28 and HPB-MLT human tumor cells.
Specificity
CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist.
Usage
Function: ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation. In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independent of TCR ligation. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride
Storage
Store at 4°C. Do not freeze. Open under aseptic conditions.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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