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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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CD28 Antibody (C‑Terminus) LS‑C117042
CD28 antibody LS-C117042 is an unconjugated rabbit polyclonal antibody to CD28 from human, mouse, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C117042-50
50 µg
Unavailable

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Anti-CD28 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunoprecipitation, Flow Cytometry, Functional Assay

Product Description

CD28 antibody LS-C117042 is an unconjugated rabbit polyclonal antibody to CD28 from human, mouse, guinea pig and other species. Validated for WB.
About CD28
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. P10747 NM_006139 NP_006130.1

CD28 Antibody, CD28 antigen Antibody, CD28 antigen (Tp44) Antibody, Tp44 Antibody, CD28 molecule Antibody


Specifications

Target
Human CD28
Host
Rabbit
Reactivity
Human, Mouse, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Bovine, Dog, Pig, Sheep (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CD28 antibody was raised against synthetic peptide from C-Terminus of human CD28 (P10747, NP_006130). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Elephant, Rabbit, Horse (100%); Rat, Sheep, Dog, Cat, Bovine, Pig, Opossum (92%); Marmoset (91%); Mouse, Guinea pig (85%).
Blocking Peptide
LS-E21611 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C117042. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human CD28
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CD28 antibody LS-C117042 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD28 antibody LS-C117042 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CD28 antibody LS-C117042 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD28 antibody LS-C117042 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CD28 antibody LS-C117042 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD28 antibody LS-C117042 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CD28 antibody LS-C117042 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD28 antibody LS-C117042 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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