Human CD275 / B7-H2 / ICOS Ligand

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhICOS Ligand.

Recognizes human ICOS Ligand. In direct ELISAs and Western Blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, rmB7-H2 or rmPD-L2.

• Western blot (1 - 2 µg/ml)
• ELISA (0.5 - 1 µg/ml)
• Neutralization

Suitable for use in Neutralization, ELISA and Western Blot. Neutralization: ~1-4 ug/ml Dependent on cell type, growth conditions, activity studied. ND50 in the presence of 20 ng/ml of anti-human CD3 and 3 ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA stimulated human T cells as an assay. ELISA: 0.5-1 ug/ml, limit B7-H2 ~5 ng/well. Western Blot: 1-2 ug/ml, limit B7-H2 ~1 ng/lane.

Lyophilized from PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75144 NCBI: AF289028 AAG01176.1