Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human CD274 / B7-H1 / PD-L1
Human CD274 / B7-H1 / PD-L1
Human (tested or 100% immunogen sequence identity)
Protein G purified
Specificity and Use
CD274 / B7-H1 / PD-L1 antibody was raised against human CD274 transfected P815 cells.
Recognizes human CD274.
Suitable for use in Flow cytometry: Neat-1:10. 10 ul labels 10^6 cells in 100ul.
PBS, pH 7.4, 1% BSA, 0.09% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. Sensitive to light.
CD274, also known as B7-H1 and PD-L1, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to interferons. CD274 has dual functions: inhibition of activated effector T cells and co-stimulation of naïve T cells.