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Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH6, FITC) LS-C58198

Note: This antibody replaces LS-C128034

Ordering

Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $340
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD274 / B7-H1 / PD-L1 Antibodies

Anti-CD274 / B7-H1 / PD-L1 Antibody (aa19-338, clone 130021) LS-C128031
Mouse Monoclonal [clone 130021] (IgG1) to Human CD274 / B7-H1 / PD-L1
Human
Flow Cytometry, ELISA
Unconjugated
Anti-CD274 / B7-H1 / PD-L1 Antibody LS-C150153
Goat Polyclonal (IgG) to Mouse CD274 / B7-H1 / PD-L1
Mouse
IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-CD274 / B7-H1 / PD-L1 Antibody (clone 27A2) LS-C179481
Mouse Monoclonal [clone 27A2] (IgG2b) to Human CD274 / B7-H1 / PD-L1
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone MIH6] (IgG2a) to Mouse CD274 / B7-H1 / PD-L1
Mouse
IHC - Frozen, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
FITC Conjugated

Details

Mouse CD274 / B7-H1 / PD-L1
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [MIH6]
Fluorescein. Also available Unconjugated.
Affinity purified
Unmodified

Applications

  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)

Specificity and Use

CD274 / B7-H1 / PD-L1 antibody was raised against mouse CD274 - transfected L5178Y cells.
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Packaging

PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Short term store at 4°C. Long term, aliquot into single use aliquots and store at -20°C. Avoid freeze-thaw cycles and prolonged exposure to light. Store undiluted.
For research use only.

About CD274 / B7-H1 / PD-L1

Q9NZQ7 NM_014143 NP_054862.1

CD274 Antibody, B7-H Antibody, B7 homolog 1 Antibody, B7-H1 Antibody, B7H1 Antibody, CD274 molecule Antibody, CD274 antigen Antibody, PDCD1 ligand 1 Antibody, PDL1 Antibody, PD-L1 Antibody, PDCD1L1 Antibody, PDCD1LG1 Antibody, Programmed death ligand 1 Antibody

CD274, also known as B7-H1 and PD-L1, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to interferons. CD274 has dual functions: inhibition of activated effector T cells and co-stimulation of naïve T cells.

Flow Cytometry

Flow Cytometry
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/6/2016

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