Mouse CD274 / B7-H1 / PD-L1
(tested or 100% immunogen sequence identity)
Also available Unconjugated
- IHC - Frozen
- Flow Cytometry (1:1)
- (applications tested for the base form of this product only)
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Mouse CD274 - transfected L5178Y cells.
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.