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Anti-CD274 / B7-H1 / PD-L1 Antibody (clone MIH5) LS-C107430

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $215
100 µg - 0.5 mg/ml $245
500 µg - 0.5 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD274 / B7-H1 / PD-L1 Antibodies

Anti-CD274 / B7-H1 / PD-L1 Antibody (aa19-338, clone 130021) LS-C128031
Mouse Monoclonal [clone 130021] (IgG1) to Human CD274 / B7-H1 / PD-L1
Human
Flow Cytometry, ELISA
Unconjugated
Anti-CD274 / B7-H1 / PD-L1 Antibody LS-C150153
Goat Polyclonal (IgG) to Mouse CD274 / B7-H1 / PD-L1
Mouse
IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-CD274 / B7-H1 / PD-L1 Antibody (clone 27A2) LS-C179481
Mouse Monoclonal [clone 27A2] (IgG2b) to Human CD274 / B7-H1 / PD-L1
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone MIH5] (IgG2a,l) to Mouse CD274 / B7-H1 / PD-L1
Mouse
IHC - Frozen, Western blot, Flow Cytometry
Unconjugated

Details

Mouse CD274 / B7-H1 / PD-L1
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a,l Monoclonal [MIH5]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Frozen
  • Western blot
  • Flow Cytometry

Specificity and Use

CD274 / B7-H1 / PD-L1 antibody was raised against mouse PDCD1LG2
The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About CD274 / B7-H1 / PD-L1

Q9NZQ7 NM_014143 NP_054862.1

CD274 Antibody, B7-H Antibody, B7 homolog 1 Antibody, B7-H1 Antibody, B7H1 Antibody, CD274 molecule Antibody, CD274 antigen Antibody, PDCD1 ligand 1 Antibody, PDL1 Antibody, PD-L1 Antibody, PDCD1L1 Antibody, PDCD1LG1 Antibody, Programmed death ligand 1 Antibody

CD274, also known as B7-H1 and PD-L1, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to interferons. CD274 has dual functions: inhibition of activated effector T cells and co-stimulation of naïve T cells.

Flow Cytometry

Flow Cytometry
Surface staining of mouse splenocytes with anti-mouse B7-H1 (MIH5) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
 

Requested From: United States
Date Requested: 12/8/2016

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